Method for Isolation, Enumeration and Confirmation of Sulphite Reducing Clostridia and Clostridium perfringens from Raw, Potable, Process and Recreational Waters by Membrane Filtration
The sample is filtered and the membrane filter placed on the surface of an agar medium containing sulphite and iron(III). The agar medium is then incubated under anaerobic conditions at either 37°C for sulphite-reducing clostridia or 44°C for Clostridium perfringens.
Methodology – Environment Agency, The Microbiology of Drinking Water (2010), Part 6 – Methods for the isolation and enumeration of sulphite-reducing clostridia and Clostridium perfringens by membrane filtration.
